Dark Field Microscopy


The dark field microscopy is a methodology for the study of the blood cells by means of an optical microscope, to which a special capacitor capable of highlighting invisible details is added. This is also one of the most frequently used methods at Ortho-Bio-Med. It is often performed during the first visit.


How does it work?

A small drop of blood from the patient’s finger is taken. The drop is immediately placed on a glass slide. After that, the sample is put under the microscope in the dark field. The patient can follow the analysis on a screen.

The first examination takes about 10-15 minutes. Subsequently, the sample is re-evaluated after 1 hour, 4-6 hours and, in some cases, even after that.


What is the utility of the dark field microscopy and what information does it provide?

It does not replace the conventional “framework hematologic test,” but it completes it.

It provides important information about the so-called “internal milieu” information, i.e. the environment in which cells live and reproduce.

Dark field microscopy allows a very significant morphological vision. The shape, size and behaviour of the blood cells may be indicative for the dynamic study of the biology of the organism, and it indicates the presence of any pathogens in the blood.

You can find information such as:

  • the size and shape of red blood cells that gives us an idea of the possible deficiencies of B vitamins and minerals;
  • the presence of symbiotic microorganisms or parasites: how to mirror the functionality of the immune system;
  • the integrity of the membranes of blood cells: index of the quality of fatty acids that make up cell membranes and thus of the need for integration;
  • Granulocyte Mobility: reactivity index to fight infections;
  • Vital blood resistance: oxidative stress test;
  • the presence of metabolic toxins: index indicating the difficulty to dispose of these toxins, which can show a possible enzyme deficiency;
  • the presence of fungi or molds: index showing the tendency to develop chronic degenerative diseases;
  • an increased number of white blood cells: acute inflammatory conditions, chronic or regression
  • the aggregation of red blood cells: tissue hypoxia index and consequent acidosis—possible correlation with circulatory problems.

Through these tests, we can better address the diagnosis, customizing the therapy as well.


What are the signs?

It is very useful as an additional diagnostic tool in patients with chronic or degenerative problems, such as:

  • Chronic pain
  • Tumours
  • Autoimmune disorders
  • Allergies
  • Cardiovascular problems


Is it also useful for therapy?

Certainly; the method serves as a means of prevention and follow-up for the performance of therapies.

In addition, after treatment, it will be very pleasant for the patient to observe the benefits of the therapies implemented.


All rights reserved – Orthobiomed 2016®